Repository of Research and Investigative Information

Repository of Research and Investigative Information

Torbat Heydariyeh University of Medical Sciences

Immunization against PR8 influenza virus with chitosan-coated ISCOMATRIX nanoparticles

(2018) Immunization against PR8 influenza virus with chitosan-coated ISCOMATRIX nanoparticles. Artificial cells, nanomedicine, and biotechnology. pp. 1-7. ISSN 2169-141X (Electronic) 2169-1401 (Linking)


Download (1MB) | Preview

Official URL:


Chitosan-coated ISCOMATRIX nanoparticles co-administrated with PR8 influenza virus were successfully developed via a lipid film hydration method to evaluate their in vivo immuniadjuvant potential in immunization against influenza. The prepared ISCOMATRIX (ISC) and chitosan-coated ISCOMATRIX (ISC-CIT) showed a particle size of 171 and 233 nm with a zeta potential of -9.47 and +5.65, respectively. Furthermore, ISC-CIT formulations were co-administered with PR8 antigen (PR8-ISC-CIT) and their immunogenicity was investigated after intranasal and intramuscular immunization of BALBc/mice. The PR8-ISC formulation elicited more IFN-gamma after intranasal or intramuscular administration compared with PR8-ISC-CIT formulation. In contrast, although PR8-ISC-CIT formulation administered by intranasal route secreted more IFN-gamma, it significantly decreased the IgG2a/IgG1 ratio and a less immune response was induced. Altogether, the ISC-adjuvanted influenza PR8 antigen could be considered as a powerful intramuscular antigen delivery system for producing a variety of prophylactic and therapeutic vaccines.

Item Type: Article
Keywords: Iscomatrix; Immune response; PR8 influenza virus; chitosan-coated ISCOMATRIX; nasal immunization
Page Range: pp. 1-7
Journal or Publication Title: Artificial cells, nanomedicine, and biotechnology
Journal Index: ISI, Pubmed, Scopus
Identification Number: 10.1080/21691401.2018.1464460
ISSN: 2169-141X (Electronic) 2169-1401 (Linking)
Depositing User: دکتر محبوبه عبداللهی

Actions (login required)

View Item View Item