Abstract

Background: Cinnamon (Cinnamomum zeylanicum) and Clove (Syzygium aromaticum) essential oils are two medicinally important plant-derived substances with a wide range of biological properties. Besides, nanoemulsion-based gels have been widely used to increase topical drug delivery and efectiveness. Methods: This study aimed to explore the anti-infammatory efect (paw edema test) and the anti-nociceptive efect (hot plate and formalin test) of nanoemulsion-based gels containing the essential oils in the animal model. Cinnamon and Clove essential oils nanoemulsions with droplet sizes of 28±6nm and 12±3nm were frst prepared. By adding carboxymethylcellulose (3.5% w/v), the nanoemulsions were then gelifed. Finally, the nanogels were characterized by ATR-FTIR analysis and were used as topical pre-treatment before induction of infammation or pain in acute and chronic analgesic experimental studies. Results: The paw edema and formalin fndings showed that the nanogels formulations possess signifcant anti-nociceptive and anti-infammatory efects. Conclusion: The prepared nanogels could be considered as analgesic drugs for inhibiting the infammation and pain of diseases.