(2020) Exposure to bis (2-ethylhexyl) phthalate causes sperm quality deteriorations, oxidative stress and down-regulates the expression of Catsper2 gene and protein in testis of mice. Medical Science. pp. 3128-3136. ISSN 2321-7359
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Abstract
Purpose: Environmental factors such as exposure to heavy metals and air pollutants are factors influencing the male fertility. The present study was conducted to determine the effect of bis (2-ethylhexyl) phthalate administration on sperm parameters, testicular histopathology, expression of Catsper2 gene and protein and oxidative stress in male adult mice. Methods: In this study, 32 male NMRI mice were randomly divided into 4 groups of control and phthalate 1-3. The control group received the solvent of bis(2ethylhexyl) phthalate (corn oil). The phthalate 1-3 groups received 50, 250, and 500 mg/kg of bis(2-ethylhexyl) phthalate for 14 days, respectively. Sperm, CatSper2 gene and protein expression, testicular histopathology and oxidative stress assessments were performed 5 weeks after the intervention. Results: Our results showed that, the administration of phthalate reduced sperm parameters as well as interstitial space and congestion with incomplete spermatogenesis in seminiferous tubules. Mice received phthalate showed a significant decrease in CatSper2 gene expression compared to the controls (p<0.05). The changes identified in CatSper2 protein expression levels were consistent with their relative mRNA level. A significant difference was found in the mean levels of malondialdehyde and superoxide dismutase enzyme between the phthalate 2 and 3 groups and the control group. A significant increase was observed in the mean thiol level in phthalate 1 (p = 0.04), phthalate 2 (p<0.001) and phthalate 3 (p<0.001) groups compared to the control group. Conclusion: The exposure to bis(2-ethylhexyl) phthalate reduces sperm quality especially sperm with tail defects and degenerative changes in testicular tissue and increases oxidative stress in male mice. A dose-dependent reduction was also observed in CatSper gene and protein expression
Item Type: | Article |
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Keywords: | Phthalate; CatSper; Sperm; Testis; Mice |
Page Range: | pp. 3128-3136 |
Journal or Publication Title: | Medical Science |
Volume: | 24 |
Number: | 105 |
ISSN: | 2321-7359 |
Depositing User: | پریسا مرادی |
URI: | http://eprints.thums.ac.ir/id/eprint/3236 |
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