(2018) Immunization against PR8 influenza virus with chitosan-coated ISCOMATRIX nanoparticles. Artificial cells, nanomedicine, and biotechnology. pp. 1-7. ISSN 2169-141X (Electronic) 2169-1401 (Linking)
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Abstract
Chitosan-coated ISCOMATRIX nanoparticles co-administrated with PR8 influenza virus were successfully developed via a lipid film hydration method to evaluate their in vivo immuniadjuvant potential in immunization against influenza. The prepared ISCOMATRIX (ISC) and chitosan-coated ISCOMATRIX (ISC-CIT) showed a particle size of 171 and 233 nm with a zeta potential of -9.47 and +5.65, respectively. Furthermore, ISC-CIT formulations were co-administered with PR8 antigen (PR8-ISC-CIT) and their immunogenicity was investigated after intranasal and intramuscular immunization of BALBc/mice. The PR8-ISC formulation elicited more IFN-gamma after intranasal or intramuscular administration compared with PR8-ISC-CIT formulation. In contrast, although PR8-ISC-CIT formulation administered by intranasal route secreted more IFN-gamma, it significantly decreased the IgG2a/IgG1 ratio and a less immune response was induced. Altogether, the ISC-adjuvanted influenza PR8 antigen could be considered as a powerful intramuscular antigen delivery system for producing a variety of prophylactic and therapeutic vaccines.
Item Type: | Article |
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Keywords: | Iscomatrix; Immune response; PR8 influenza virus; chitosan-coated ISCOMATRIX; nasal immunization |
Page Range: | pp. 1-7 |
Journal or Publication Title: | Artificial cells, nanomedicine, and biotechnology |
Journal Index: | ISI, Pubmed, Scopus |
Identification Number: | 10.1080/21691401.2018.1464460 |
ISSN: | 2169-141X (Electronic) 2169-1401 (Linking) |
Depositing User: | دکتر محبوبه عبداللهی |
URI: | http://eprints.thums.ac.ir/id/eprint/317 |
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