Repository of Research and Investigative Information

Repository of Research and Investigative Information

Torbat Heydariyeh University of Medical Sciences

Inducible nitric oxide synthase inhibitor, aminoguanidine improved Ki67 as a marker of neurogenesis and learning and memory in juvenile hypothyroid rats

(2020) Inducible nitric oxide synthase inhibitor, aminoguanidine improved Ki67 as a marker of neurogenesis and learning and memory in juvenile hypothyroid rats. International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience. ISSN 1873-474X (Electronic) 0736-5748 (Linking)

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Official URL: http://www.ncbi.nlm.nih.gov/pubmed/32479691

Abstract

INTRODUCTION: In the present study the effect of inducible nitric oxide synthase (iNOS) inhibitor, aminoguanidine (AG) on neurogenesis indicators, learning and memory and oxidative stress status in juvenile hypothyroid (Hypo) rats was evaluated. METHOD: The studied groups were including: (1) Control, (2) hypothyroid (Hypo), (3-5) Hypo-AG 10, Hypo-AG 20 and Hypo-AG 30. Hypothyroidism was induced in the groups 2-5 by adding propylthiouracil (PTU) in drinking water (0.05). AG (10, 20 or 30 mg/kg) was daily injected intraperitoneally (IP) in the groups 3-5. The rats of the groups 1 and 2 were injected by saline instead of AG. After 6 weeks treatment, Morris water maze (MMW) and passive avoidance (PA) tests were done. Deep anesthesia was then induced and the brain tissue was excised for biochemical parameters measuring. RESULTS: Ki67 as a maker of neurogenesis and thiol, superoxide dismutase (SOD and catalase (CAT as oxidative stress indicators were decreased in the brain of Hypo group, whereas malondialdehyde (MDA) and nitric oxide (NO) metabolites were enhanced. AG improved Ki67, thiol, CAT and SOD while decreased MDA and NO metabolites. The escape latency in the MWM test increased in the Hypo group. The spending time in the target quadrant in the probe test of MWM and step-through latency in the PA test in the Hypo group was lower than control group. AG reversed all the negative behavioral effects of hypothyroidism. CONCLUSION: These results revealed that AG improved neurogenesis, learning and memory impairments and oxidative imbalance in the brain juvenile Hypo rats.

Item Type: Article
Keywords: Aminoguanidine; Hypothyroidism; Learning; Memory; ki67; nitric oxide.
Journal or Publication Title: International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience
Journal Index: Pubmed, Scopus
Identification Number: 10.1002/jdn.10042
ISSN: 1873-474X (Electronic) 0736-5748 (Linking)
Depositing User: دکتر محبوبه عبداللهی
URI: http://eprints.thums.ac.ir/id/eprint/2062

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