Repository of Research and Investigative Information

Repository of Research and Investigative Information

Torbat Heydariyeh University of Medical Sciences

Investigation of blood and urine malondialdehyde levels in mice exposed to silica dust

(2019) Investigation of blood and urine malondialdehyde levels in mice exposed to silica dust. Open Biochemistry Journal. pp. 32-36. ISSN 1874091X

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Background and Objectives: Occupational exposure to silica dust can lead to biochemical damage. Malondialdehyde (MDA) can be considered as a primary marker for measuring the level of oxidative stress in a living organism. This study was conducted in order to evaluate the level of MDA in blood and urine of mice exposed to silica dust. Material and Methods: In this experimental study, 72 mice (BALB/c) were randomly allocated to five exposed groups and 1 control group. Exposure of mice to pure 99 silica dust was done in closed containers. Blood sampling was performed from the heart of mice and urine sampling fulfilled by insertion into a metabolic cage. The RAO et al. method was used to measure MDA. Results: The highest level of plasma MDA in group 1 in the 4th month was 8.4±0.41 nmol/l and the lowest level of MDA was 1.3±0.2 nmol/l in the third sampling in the control group, also the highest amount of urine MDA in the first and second groups and 4 months after exposure was 1.16±0.51 nmol/l, and the lowest in the control group and in the third sampling was 0.48±0.06 nmol/l. A significant difference was found between the levels of MDA in all exposed groups at different times except for the 5th group with the lowest concentration (P < 0.05). Conclusion: MDA in blood and urine could be proposed as a good biomarker for the evaluation of biochemical damages caused by silica dust. Measuring MDA is also a simple and inexpensive method that does not require complex equipment and can be used as an early detection test for biochemical damages caused by silica. © 2019 Gholami et al.

Item Type: Article
Keywords: malonaldehyde; silicon dioxide, animal experiment; Article; Bagg albino mouse; blood sampling; controlled study; heart; mouse; nonhuman; urine level; urine sampling
Page Range: pp. 32-36
Journal or Publication Title: Open Biochemistry Journal
Journal Index: Scopus
Volume: 13
Number: 1
Publisher: Bentham Science Publishers B.V.
Identification Number: 10.2174/1874091X01913010032
ISSN: 1874091X
Depositing User: دکتر محبوبه عبداللهی

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