(2019) The Effect of Diazinon on Cell Proliferation and Apoptosis in Testicular Tissue of Rats and The Protective Effect of Vitamin E. International journal of fertility & sterility. pp. 154-160. ISSN 2008-076X (Print) 2008-0778 (Linking)
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Abstract
Background: Diazinon (DZN) is an organophosphate pesticide, and nowadays this pesticide is mostly used in agriculture. In this study, we analyzed the effects of DZN and vitamin E (Vit E) on apoptosis and the proliferation of germ cells in rat testis. Materials and Methods: In this experimental study, 30 male Wistar rats were divided into five groups (n=6 per group) consisting of control, sham (received olive oil), experimental group i (60 mg/kg DZN), experimental group ii (60 mg/kg DZN and 200 mg/kg Vit E), and experimental group iii (200 mg/kg Vit E). After six weeks, left testis of rats was removed for the detection of proliferative cell nuclear antigen (PCNA) and terminal deoxynucleotidyl transferase end-labeling (TUNEL). Results: Compared with the control group, DZN in the experimental group i decreased the number of PCNA-positive cells and increased the number of TUNEL-positive cells (P<0.001). Vit E improved detrimental changes by the decrease in the rate of apoptosis and the increase in the proliferation of testicular germ cells (P<0.001). Conclusion: Vit E can decrease the number of TUNEL-positive cells and increase the number of PCNA-positive cells by the neutralization of the toxicity caused by DZN in the testicular tissue.
Item Type: | Article |
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Keywords: | Apoptosis; Diazinon; Proliferation; Testis; Vitamin E |
Page Range: | pp. 154-160 |
Journal or Publication Title: | International journal of fertility & sterility |
Journal Index: | ISI, Pubmed |
Volume: | 13 |
Number: | 2 |
Identification Number: | 10.22074/ijfs.2019.5612 |
ISSN: | 2008-076X (Print) 2008-0778 (Linking) |
Depositing User: | دکتر محبوبه عبداللهی |
URI: | http://eprints.thums.ac.ir/id/eprint/1223 |
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