(2019) Aminoguanidine affects systemic and lung inflammation induced by lipopolysaccharide in rats. Respiratory research. p. 96. ISSN 1465993X
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Abstract
BACKGROUND: Nitric oxide is a mediator of potential importance in numerous physiological and inflammatory processes in the lung. Aminoguanidine (AG) has been shown to have anti-inflammation and radical scavenging properties. This study aimed to investigate the effects of AG, an iNOS inhibitor, on lipopolysaccharide (LPS)-induced systemic and lung inflammation in rats. METHODS: Male Wistar rats were divided into control, LPS (1�mg/kg/day i.p.), and LPS groups treated with AG 50, 100 or 150�mg/kg/day�i.p. for five weeks. Total nitrite concentration, total and differential white blood cells (WBC) count, oxidative stress markers, and the levels of IL-4, IFN-γ, TGF-β1, and PGE2 were assessed in the serum or bronchoalveolar lavage fluid (BALF). RESULTS: Administration of LPS decreased IL-4 level (p�<�0.01) in BALF, total thiol content, superoxide dismutase (SOD) and catalase (CAT) activities (p�<�0.001) in BALF and serum, and increased total nitrite, malondialdehyde (MDA), IFN-γ, TGF-β1 and PGE2 (p�<�0.001) concentrations in BALF. Pre-treatment with AG increased BALF level of IL-4 and total thiol as well as SOD and CAT activities (p�<�0.05 to p�<�0.001), but decreased BALF levels of total nitrite, MDA, IFN-γ, TGF-β1, and PGE2 (p�<�0.01 to p�<�0.001). AG treatment decreased total WBC count, lymphocytes and macrophages in BALF (p�<�0.01 to p�<�0.001) and improved lung pathological changes including interstitial inflammation and lymphoid infiltration (p�<�0.05 to p�<�0.001). CONCLUSIONS: AG treatment reduced oxidant markers, inflammatory cytokines and lung pathological changes but increased antioxidants and anti-inflammatory cytokines. Therefore, AG may play a significant protective role against inflammation and oxidative stress that cause lung injury.
Item Type: | Article |
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Keywords: | Aminoguanidine; Inflammation; Lipopolysaccharide; Lung injury; Nitric oxide |
Page Range: | p. 96 |
Journal or Publication Title: | Respiratory research |
Journal Index: | ISI, Pubmed, Scopus |
Volume: | 20 |
Number: | 1 |
Publisher: | NLM (Medline) |
Identification Number: | 10.1186/s12931-019-1054-6 |
ISSN: | 1465993X |
Depositing User: | دکتر محبوبه عبداللهی |
URI: | http://eprints.thums.ac.ir/id/eprint/1163 |
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